All lanes : Anti-14-3-3 epsilon antibody (ab137854) at 1/5000 dilutionLane 1 : H1299 whole cell lysateLane 2 : Hela S3 whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of parafin-embedded A549 xenograft tissue labelling 14-3-3 epsilon with ab137854 at 1/500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa cells labelling 14-3-3-epsilon with ab137854 at 1/200 dilution. The image in the lower panel is costained with Hoechst 33342.
Anti-14-3-3 epsilon antibody (ab137854) at 1/10000 dilution + mouse brain whole cell lysate at 20 µg