Anti-14-3-3 epsilon antibody - Carboxyterminal end
| Name | Anti-14-3-3 epsilon antibody - Carboxyterminal end |
|---|---|
| Supplier | Abcam |
| Catalog | ab40117 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Sheep, Rabbit, Chicken, Bovine, Human, Mammalian |
| Antigen | Synthetic peptide from C terminus |
| Description | Rabbit Polyclonal |
| Gene | YWHAE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab40117 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40117, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-14-3-3 epsilon antibody - Carboxyterminal end (ab40117) at 1/500 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
14-3-3epsilon mediates the cell fate decision-making pathways in response of - 14-3-3epsilon mediates the cell fate decision-making pathways in response of
Tang S, Bao H, Zhang Y, Yao J, Yang P, Chen X. PLoS One. 2013;8(3):e55268.
Proteomic profiling in an animal model of acute pancreatitis. - Proteomic profiling in an animal model of acute pancreatitis.
Fetaud V, Frossard JL, Farina A, Pastor CM, Buhler L, Dumonceau JM, Hadengue A, Hochstrasser DF, Lescuyer P. Proteomics. 2008 Sep;8(17):3621-31.