Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab118877at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Heart (formalin-fixed, paraffin-embedded) stained with ab118877 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
All lanes : Anti-14-3-3 gamma antibody [J3H10] (ab118877) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : HeLa cell lysateLysates/proteins at 30 µg per lane.Secondarygoat anti-mouse secondary antibody conjugated to HRP developed using the ECL technique