All lanes : Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : A549 cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Flow cytometric analysis of permeablized A549 cells using ab124909 at 1/500 dilution (red) or a rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD