Anti-160 kD Neurofilament Medium antibody - Neuronal Marker
| Name | Anti-160 kD Neurofilament Medium antibody - Neuronal Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab9034 |
| Prices | $390.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-F IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Rat, Bovine, Human, Mouse, Cat, Pig, Bird |
| Antigen | Recombinant C-terminal fragment (Rat) |
| Description | Rabbit Polyclonal |
| Gene | NEFM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
The image illustrates the high definition dendritic staining obtained after protease pretreatment. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/20000 dilution + whole rat cerebellum homogenate
ICC/IF image of ab9034 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9034, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/5000 dilutionLane 1 : Cow 160 kD Neurofilament Medium full length protein (ab73759) at 0.1 µgLane 2 : Cow 160 kD Neurofilament Medium full length protein (ab73759) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
Product References
Loss of Usp9x disrupts cortical architecture, hippocampal development and - Loss of Usp9x disrupts cortical architecture, hippocampal development and
Stegeman S, Jolly LA, Premarathne S, Gecz J, Richards LJ, Mackay-Sim A, Wood SA. PLoS One. 2013 Jul 5;8(7):e68287.
Dysregulation of insulin signaling, glucose transporters, O-GlcNAcylation, and - Dysregulation of insulin signaling, glucose transporters, O-GlcNAcylation, and
Deng Y, Li B, Liu Y, Iqbal K, Grundke-Iqbal I, Gong CX. Am J Pathol. 2009 Nov;175(5):2089-98.
Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and - Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and
Deng Y, Li B, Liu F, Iqbal K, Grundke-Iqbal I, Brandt R, Gong CX. FASEB J. 2008 Jan;22(1):138-45. Epub 2007 Aug 8.
Differential modulation of neurite growth by the S- and the L-forms of bag1, a - Differential modulation of neurite growth by the S- and the L-forms of bag1, a
Frebel K, Wiese S, Funk N, Puhringer D, Sendtner M. Neurodegener Dis. 2007;4(2-3):261-9.
Axonal growth and guidance defects in Frizzled3 knock-out mice: a comparison of - Axonal growth and guidance defects in Frizzled3 knock-out mice: a comparison of
Wang Y, Zhang J, Mori S, Nathans J. J Neurosci. 2006 Jan 11;26(2):355-64.
Distinct and overlapping alterations in motor and sensory neurons in a mouse - Distinct and overlapping alterations in motor and sensory neurons in a mouse
Jablonka S, Karle K, Sandner B, Andreassi C, von Au K, Sendtner M. Hum Mol Genet. 2006 Feb 1;15(3):511-8. Epub 2006 Jan 5.