Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker
| Name | Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab8135 |
| Prices | $401.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF ICC/IF IHC-P IHC-F WB |
| Species Reactivities | Mouse, Rat, Bovine, Cat, Human, Pig |
| Antigen | Recombinant fragment (Cow) |
| Description | Rabbit Polyclonal |
| Gene | NEFH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
The image showing results obtained after protease pretreatment, illustrating high definition neurofilament staining. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
ab8135 at 1/500 staining aged muscle tissue sections from Rat by IHC-Fr.The tissue section was permeabilized with Triton X100 and blocked with 10% serum for 30 minutes at 25°C.The primary antibody was incubated for 1 hour at 25°C.Muscle was stained without fixation and post-fixed in 10% NBF following staining for 1h.Green = alpha bungarotoxin stained post-synaptic neuromuscular junction. Red = ab8135 staining 200kD NeurofilamentSee Abreview
ab8135 staining 200 kD Neurofilament Heavy in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was fixed with formaldehyde, blocked with 5% serum for 1 hour at 25°C and permeabilzed with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500 in diluent) for 12 hours at 25°C. An Alexa Fluor®555 goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.See Abreview
Product References
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