Anti-5 Lipoxygenase antibody [EP6072(2)]

• Supplier: Abcam
• Catalog: ab169755
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP6072(2)
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Dog
• Antigen: A synthetic peptide corresponding to residues in Human 5 Lipoxygenase (P09917)
• Description: Rabbit Monoclonal
• Gene: ALOX5
• Conjugate: Unconjugated

Product images

ab169755 staining 5 Lipoxygenase in the Raw264.7 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/100). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab169755 staining 5 Lipoxygenase in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

All lanes : Anti-5 Lipoxygenase antibody [EP6072(2)] (ab169755) at 1/1000 dilutionLane 1 : Rat heart lysateLane 2 : Rat kidney lysateLane 3 : Rat spleen lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-5 Lipoxygenase antibody [EP6072(2)] (ab169755) at 1/1000 dilution + K562 cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

+ Human fetal thymus at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunofluorescent analysis of HUVEC cells labeling 5 Lipoxygenase with ab169755, unpurified, at 1/100 dilution.

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling 5 Lipoxygenase with ab169755, unpurified, at 1/100 dilution.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 5 Lipoxygenase with ab169755, unpurified, at 1/100 dilution.

All lanes : Anti-5 Lipoxygenase antibody [EP6072(2)] (ab169755) at 1/1000 dilution (unpurified)Lane 1 : Fetal thymus lysateLane 2 : K562 lysateLane 3 : HUVEC lysateLysates/proteins at 10 µg per lane.