PTTG2-Antibody-Center
| Name | PTTG2-Antibody-Center |
|---|---|
| Supplier | Abgent, a WuXi AppTec company |
| Catalog | AP16911c |
| Prices | $99.00, $295.00 |
| Sizes | 80 µl, 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Applications | WB ICC/IF ELISA |
| Species Reactivities | Human |
| Antigen | 56-84 aa |
| Purity/Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Description | Rabbit Polyclonal |
| Gene | PTTG2 |
| Supplier Page | Shop |
Product images
PTTG2 Antibody (Center) (Cat. #AP16911c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PTTG2 antibody detected the PTTG2 protein (arrow).
Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody (Center)(Cat#AP16911c).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTTG2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). PTTG2 immunoreactivity is localized to Cytoplasm significantly.
Anti-PTTG2 Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.