All lanes : Anti-5HT3A receptor antibody (ab45314) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
IHC image of 5HT3A staining in human hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45314, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.