β-Actin (13E5) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 4970
• Prices: $99.00, $256.00, $637.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 13E5
• Applications: WB IHC-P IHC-F ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey, Bovine, Pig, Hamster, Chicken, Dog, Horse
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human β-actin protein.
• Description: Rabbit Monoclonal
• Gene: ACTB

Product images

Western blot analysis of cell extracts from various cell lines using beta-Actin (13E5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human heart using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of cardiac actin.

Immunohistochemical analysis of paraffin-embedded human leiomyoma using beta-Actin (13E5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using β-actin (13E5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using beta-Actin (13E5) Rabbit mAb in the presence of control peptide (left) or beta-Actin Blocking Peptide #1025 (right).

Immunohistochemical analysis of paraffin-embedded human skeletal muscle using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of skeletal muscle actin.

Immunohistochemical analysis of frozen H1650 xenograft showing membrane and cytoplasmic localization using β-Actin (13E5) Rabbit mAb.

Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of NIH/3T3 cells using β-Actin (13E5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).