ICC/IF image of ab101913 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101913 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab101913 at 1/200 dilution staining 5HT7 Receptor in Rat spinal cord by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). ab101913 was incubated overnight at 4°C and developed with DAB/Ni.
ab101913 at 1/200 dilution staining 5HT7 Receptor in Rat spinal cord by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). ab101913 was incubated overnight at 4°C and developed with DAB/Ni.