Anti-67kDa Laminin Receptor antibody [EPR8469]

• Supplier: Abcam
• Catalog: ab133645
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR8469
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)
• Description: Rabbit Monoclonal
• Gene: RPSA
• Conjugate: Unconjugated

Product images

Overlay histogram showing MCF7 cells stained with ab133645 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133645, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-67kDa Laminin Receptor antibody [EPR8469] (ab133645) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : C6 cell lysateLane 5 : RAW 264.7 cell lysateLane 6 : PC-12 cell lysateLane 7 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling 67kDa Laminin Receptor with ab133645 at 1/100 dilution.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labellig 67kDa Laminin Receptor with ab133645 at 1/100 dilution.

All lanes : Anti-67kDa Laminin Receptor antibody [EPR8469] (ab133645) at 1/1000 dilutionLane 1 : Mouse intestinal mucosa whole tissue lysateLane 2 : Mouse intestinal mucosa whole tissue lysateLane 3 : Mouse intestinal mucosa whole tissue lysateLysates/proteins at 30 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/2500 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.