All lanes : Anti-67kDa Laminin Receptor antibody [EPR8469] (HRP) (ab197712) at 1/5000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.