HLA-F Antibody (Center) (Cat. #AP5881c) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HLA-F antibody detected the HLA-F protein (arrow).
HLA-F Antibody (Center) (Cat. #AP5881c) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-HLA-F Antibody (Center) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.