Western blot analysis of lysate from A431 cell line, using RNF208 Antibody (Center)(Cat. #AP5743c). AP5743c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
RNF208 Antibody (Center) (Cat. #AP5743c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RNF208 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
RNF208 Antibody (Center) (Cat. #AP5743c) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.