Western blot analysis of EHD3 Antibody (N-term) (Cat. #AP7458a) in mouse lung tissue lysates (35ug/lane).EHD3 (arrow) was detected using the purified Pab.
EHD3 Antibody (N-term) (Cat. #AP7458a) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EHD3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
EHD3 Antibody (N-term) (Cat. #AP7458a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-EHD3 Antibody (N-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.