Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with YARS2 antibody (C-term) (Cat.#AP7838b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes : Anti-YARS2 Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.