Western blot analysis of ANO5 Antibody (C-term) (Cat. #AP8580b) in mouse spleen tissue lysates (35ug/lane). ANO5 (arrow) was detected using the purified Pab.
ANO5 Antibody (C-term) (Cat. #AP8580b) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of theANO5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes : Anti-ANO5 Antibody (C-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 107 kDa Blocking/Dilution buffer: 5% NFDM/TBST.