Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CCHCR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of widr cells using CCHCR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CCHCR1 Antibody (Center) (Cat. #AP8528c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green) .DAPI was used to stain the cell nuclear (blue).
Anti-CCHCR1 Antibody (Center) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.