Western blot analysis of lysate from T47D cell line, using CYP27B1 Antibody (C-term)(Cat. #AP9056b). AP9056b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot analysis of CYP27B1 Antibody (C-term) (Cat. #AP9056b) in mouse kidney tissue lysates (35ug/lane). CYP27B1 (arrow) was detected using the purified Pab.
CYP27B1 Antibody (C-term) (Cat. #AP9056B)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP27B1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
CYP27B1 Antibody (C-term) (Cat. #AP9056b) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-CYP27B1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: T47D whole cell lysate Lane 3: Rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-CYP27B1 Antibody (C-term) at 1:2000 dilution Lane 1: Human kidney lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.