Western blot analysis of immunoprecipitation pellet from A431 lysate immunoprecipitated using ab188872 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-ACADVL antibody [EPR15107(B)] (ab188872) at 1/1000 dilution + 293 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ACADVL with ab188872 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling ACADVL with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACADVL with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
All lanes : Anti-ACADVL antibody [EPR15107(B)] (ab188872) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution