Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]

• Supplier: Abcam
• Catalog: ab109368
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4971
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues at the N-terminus of Human Acetyl Coenzyme A carboxylase alpha
• Description: Rabbit Monoclonal
• Gene: ACACA
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab109368 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109368, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution

Immunofluorescent staining of 293 cells using ab109368 at 1/100 dilution

Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using ab109368 at 1/250 dilution.

Immunohistochemical analysis of paraffin-embedded brain tissue using ab109368 at 1/250 dilution.