All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Molt4 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/50000 dilution + Jurkat cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling ACF1 / BAZ1A with ab187670 at 1/100 dilution. Prediluted (Ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Antigen retrieval: Perform heat mediated antigen retrieval using EDTA Buffer, pH 9. Counter stain: Hematoxylin.
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ACF1 / BAZ1A with ab187670 at 1/100 dilution. Prediluted (Ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Antigen retrieval: Perform heat mediated antigen retrieval using EDTA Buffer, pH 9. Counter stain: Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ACF1 / BAZ1A with ab187670 at 1/250 (red) and DAPI staining (blue). Secondary aantibody: goat anti rabbit IgG(Alexa Fluor® 555).