All lanes : Anti-Acid Phosphatase antibody [EPR9839] (ab166896) at 1/1000 dilutionLane 1 : Human placenta lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP antibody at 1/2000 dilution
Flow cytometric analysis of permeabilized HeLa cells labeling Acid Phosphatase using ab166896 at 1/10 dilution (red) compared to staining with a rabbi IgG negative control antibody (green).
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166896 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.