Flow cytometric analysis of K562 cells labeling Acid sphingomyelinase with ab170579 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling Acid sphingomyelinase with ab170579 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-Acid sphingomyelinase antibody - C-terminal (ab170579) at 1/100 dilution + K562 cell lysate at 35 µg