Anti-Actin antibody [AC-40]

• Supplier: Abcam
• Catalog: ab11003
• Prices: $401.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: AC-40
• Applications: DB WB ELISA IHC-F IHC-P IHC-F ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea Pig, Hamster, Bovine, Dog, Human, Pig, Xenopus, Fish
• Antigen: Synthetic peptide within Human Actin aa 365-375 (C terminal)
• Description: Mouse Monoclonal
• Gene: ACTA1
• Conjugate: Unconjugated

Product images

ab11003 staining Actin in Human SW480 cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 2 hours at 25°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 568 (red) used at a 1/100 dilution.See Abreview

Immunohistochemical analysis of frozen Human tongue tissue, using ab11003.

ab11003 staining Human fibroblast cells by ELISA (Sandwich-capture). Cells were blocked in 1% BSA for 1 hour at 25°C. The  primary antibody ab11003 (Capture antibody) was diluted 1/3000 and incubated with sample for 12 hours at 4°C. ab5694 was used as secondary and a HRP conjugated goat polyclonal to mouse, diluted 1/1000 was used as detection antibody.See Abreview

Product References

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