Anti-Actin antibody [EPR16769]

• Supplier: Abcam
• Catalog: ab179467
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16769
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Chicken, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Actin aa 50-150
• Description: Rabbit Monoclonal
• Gene: ACTA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysatesLane 3 : Human skeletal muscle lysateLane 4 : Human fetal spleen lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/5000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal heart lysateLane 3 : Human fetal kidney lysateLane 4 : Human fetal spleen lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilutionLane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates)Lane 2 : Human cardiac muscle lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Actin antibody [EPR16769] (ab179467) at 1/20000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Mouse spleen lysateLane 5 : Rat brain lysateLane 6 : Rat heart lysateLane 7 : Rat kidney lysateLane 8 : Rat spleen lysateLane 9 : C6 (Rat glial tumor cells) whole cell lysatesLane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling Actin with ab179467 at 1/50 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/200 dilution (green). Cytoplasm staining on NIH/3T3 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary) at 1/500 dilution (red).The negative controls are as follows;1. ab179467 at 1/50 dilution followed by ab150120 (goat anti-mouse AlexaFluor®594 secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (goat anti-rabbit Alexa Fluor®488 (IgG H&L) at 1/200 dilution.

Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on smooth muscle cells is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining is observed. Counter stained with Hematoxylin.Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Actin with ab179467 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Actin was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab179467 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab179467 at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Lane 1: NIH/3T3 whole cell extract. Lane 2: PBS instead of NIH/3T3 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.