Anti-Actin antibody [MAC 237]
| Name | Anti-Actin antibody [MAC 237] |
|---|---|
| Supplier | Abcam |
| Catalog | ab50591 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | MAC 237 |
| Applications | IHC-P ICC/IF ICC/IF Electron microscopy |
| Species Reactivities | Rat, Human, Drosophila |
| Antigen | Flight muscle extract from Lethocerus indicus (Waterbug) |
| Description | Rat Monoclonal |
| Gene | ACTA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Actin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50591, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
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