Anti-activated Notch1 antibody

• Supplier: Abcam
• Catalog: ab8925
• Prices: $400.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P IHC-F ICC/IF ICC/IF WB FC
• Species Reactivities: Mouse, Human, Rat
• Antigen: Peptide: VLLSRKRRRQHGQC (aa1755-1767), human N-terminal sequence of the cleaved N intracellular domain (NICD)
• Description: Rabbit Polyclonal
• Gene: NOTCH1
• Conjugate: Unconjugated

Product images

ab8925 staining actived Notch1 in the NIH3T3 fibroblast cell line from Mouse embryo by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal(1/500) was used as the secondary antibody.See Abreview

Stacey Huppert, Ma. Xenia U. Garcia (lab of Raphael Kopan)Rabbit polyclonal (ab8925) at 1/500, against myc-tagged transiently transfected mouse Notch constructs in 293 cells.Lane M: Mol wt markersLane 1 No transfectionLane 2 N1 (mouse deleted extracellular domain)-mycLane 3 N1 (mouse intracellular domain)-mycLane 4 N2 (mouse deleted extracellular domain)-mycLane 5 N2 (mouse intracellular domain)-mycLane 6 N3 (mouse deleted extracellular domain)-mycLane 7 N3 (mouse intracellular domain)-mycLane 8 N4 (mouse deleted extracellular domain)-mycLane 9 N4 (mouse intracellular domain)-mycLane 10 N1 (mouse deleted extracellular domain)(V to G)-myc Rabbit polyclonal (ab8925) at 1/500, against myc-tagged transiently transfected mouse Notch constructs in 293 cells. Lane M: Mol wt markers Lane 1 No transfection Lane 2 N1 (mouse deleted extracellular domain)-myc Lane 3 N1 (mouse intracellular domain)-myc Lane 4 N2 (mouse deleted extracellular domain)-myc

ab8925 (1:500) diluted staining activated Notch1 in human ovarian carcinoma using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of activated Notch 1 in nuclear/nucleolar compartments of the ovarian cortex.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ab8925 staining activated Notch 1 in murine dermis (including follicle) by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 10% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor® conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).See Abreview

Product References

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Clinical impact of de-regulated Notch-1 and Notch-3 in the development and - Clinical impact of de-regulated Notch-1 and Notch-3 in the development and

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