Overlay histogram showing HEK293 cells stained with ab109300 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109300, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilutionLane 1 : Human brain tissue lysateLane 2 : Human fetal kidneyLane 3 : SH-SY5Y cell lysateLane 4 : U87-MG cell lysateLane 5 : Human fetal liverLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2281_Activin-A-Receptor-Type-IB-Primary-antibodies-ab109300-1.JPG)
All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilutionLane 1 : Human brain tissue lysateLane 2 : Human fetal kidneyLane 3 : SH-SY5Y cell lysateLane 4 : U87-MG cell lysateLane 5 : Human fetal liverLysates/proteins at 10 µg per lane.
ab109300, at 1/500 dilution, staining Activin A receptor type IB in Human kidney tissue by immunohistochemistry.
Alexa Fluor 488 goat anti-rabbit IgG was used as the secondary antibody. The cells (SH-SY5Y) were fixed with 2% paraformaldehyde for 20 min.