Immunohistochemical analysis of paraffin-embedded R. brain section using NSF Antibody (Cat#AN1110). AN1110 was diluted at 1:500 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. brain section using NSF Antibody (Cat#AN1110). AN1110 was diluted at 1:500 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of PC-12 cells using Park7 (DJ-1) Antibody(green, Cat#AN1110) compared to an isotype control of rabbit IgG(blue). AN1110 was diluted at 1:500 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from mouse C2C12 cell line, rat brain and mouse brain tissue lysate(from left to right), using Nsf Antibody(Cat. #AN1110). AN1110 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of rat caudate lysate showing specific labeling of the~75k NSF protein.