Flow cytometric analysis of Hela cells using NDUFB4 Antibody (N-term)(green, Cat#AP20608a) compared to an isotype control of rabbit IgG(blue). AP20608a was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Fluorescent image of Hela cells stained with NDUFB4 Antibody (N-term)(Cat#AP20608a). AP20608a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. skin section using NDUFB4 Antibody (N-term)(Cat#AP20608a). AP20608a was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from HepG2, PC-3, Ramos, U-251 MG cell line and human heart tissue lusyte(from left to right), using NDUFB4 Antibody (N-term)(Cat. #AP20608a). AP20608a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.