MDM2 (D-12): sc-5304. Western blot analysis of MDM2 expression in Jurkat whole cell lysate.
MDM2 (D-12) PE: sc-5304 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-2866.
MDM2 siRNA (h): sc-29394. Western blot analysis of MDM2 expression in non-transfected control (A) and MDM2 siRNA transfected (B) Jurkat cells. Blot probed with MDM2 (D-12): sc-5304. α-actinin (H-2): sc-17829 used as specificity and loading control.
MDM2 (D-12) Alexa Fluor 488: sc-5304 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-3890.
MDM2 (D-12): sc-5304. Western blot analysis of MDM2 expression in untreated (A) and ()-Nutlin-3 (sc-222086) treated (B) Hep G2 whole cell lysates. Note upregulation of MDM2 expression in lane B.