Anti-ADA2 beta antibody
| Name | Anti-ADA2 beta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57953 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P FC |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Recombinant fragment, corresponding to amino acids 2-111 of Human ADA2 beta |
| Description | Mouse Monoclonal |
| Gene | TADA2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing K562 cells stained with ab57953 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57953, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2512_ab57953-3-ab57953FC.jpg)
Overlay histogram showing K562 cells stained with ab57953 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57953, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab57953 (1.5µg/ml) staining ADA2 beta in formalin-fixed paraffin-embedded human stomach sections.
Product References
Role for human mediator subunit MED25 in recruitment of mediator to promoters by - Role for human mediator subunit MED25 in recruitment of mediator to promoters by
Sela D, Conkright JJ, Chen L, Gilmore J, Washburn MP, Florens L, Conaway RC, Conaway JW. J Biol Chem. 2013 Sep 6;288(36):26179-87.
Endoplasmic reticulum stress-responsive transcription factor ATF6alpha directs - Endoplasmic reticulum stress-responsive transcription factor ATF6alpha directs
Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC. J Biol Chem. 2012 Jun 29;287(27):23035-45.
Activator-Mediator binding regulates Mediator-cofactor interactions. - Activator-Mediator binding regulates Mediator-cofactor interactions.
Ebmeier CC, Taatjes DJ. Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11283-8. doi: