All lanes : Anti-ADAM10 antibody (ab84595) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : THP1 (Human acute monocytic leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-ADAM10 antibody (ab84595) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : Spleen (Mouse) Tissue Lysate Lane 3 : Liver (Mouse) Tissue LysateLane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 5 : Spleen (Rat) Tissue LysateLane 6 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![ADAM10 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to ADAM10 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84595.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 61kDa; ADAM10, Non-specific bands: 70 and 75kDa, We are unsure as to the identity of these extra bands.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2541_ADAM10-Primary-antibodies-ab84595-10.jpg)
ADAM10 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to ADAM10 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84595.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 61kDa; ADAM10, Non-specific bands: 70 and 75kDa, We are unsure as to the identity of these extra bands.