Anti-ADAM10 antibody [mAbcam 73402]
| Name | Anti-ADAM10 antibody [mAbcam 73402] |
|---|---|
| Supplier | Abcam |
| Catalog | ab73402 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | mAbcam 73402 |
| Applications | FC WB |
| Species Reactivities | Human, Mouse, Rat, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human ADAM10 |
| Description | Mouse Monoclonal |
| Gene | ADAM10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-ADAM10 antibody [mAbcam 73402] (ab73402) at 10 µg/ml + Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) at 20 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2549_ADAM10-Primary-antibodies-ab73402-1.jpg)
Anti-ADAM10 antibody [mAbcam 73402] (ab73402) at 10 µg/ml + Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) at 20 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HeLa cells stained with ab73402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73402, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2550_ADAM10-Primary-antibodies-ab73402-3.jpg)
Overlay histogram showing HeLa cells stained with ab73402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73402, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.