Anti-ADAM10 antibody - Cytoplasmic domain
| Name | Anti-ADAM10 antibody - Cytoplasmic domain |
|---|---|
| Supplier | Abcam |
| Catalog | ab39177 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P WB ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Bovine, Human, Rat |
| Antigen | Synthetic peptide based on the Cytoplasmic domain of human ADAM10, within the last 30 C-terminus amino acids of the 748 amino acid human sequence |
| Description | Rabbit Polyclonal |
| Gene | ADAM10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Methanol fixed HeLs cells stained with ab39177 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview
IHC image of ab39177 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39177, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Adam10 mediates the choice between principal cells and intercalated cells in the - Adam10 mediates the choice between principal cells and intercalated cells in the
Guo Q, Wang Y, Tripathi P, Manda KR, Mukherjee M, Chaklader M, Austin PF, Surendran K, Chen F. J Am Soc Nephrol. 2015 Jan;26(1):149-59.
A disintegrin and metalloprotease 10 activity sheds the ectodomain of the amyloid - A disintegrin and metalloprotease 10 activity sheds the ectodomain of the amyloid
Cong R, Li Y, Biemesderfer D. Am J Physiol Cell Physiol. 2011 Jun;300(6):C1366-74. doi:
VIP36 protein is a target of ectodomain shedding and regulates phagocytosis in - VIP36 protein is a target of ectodomain shedding and regulates phagocytosis in
Shirakabe K, Hattori S, Seiki M, Koyasu S, Okada Y. J Biol Chem. 2011 Dec 16;286(50):43154-63.
Anti-aging properties of melatonin in an in vitro murine senescence model: - Anti-aging properties of melatonin in an in vitro murine senescence model:
Tajes M, Gutierrez-Cuesta J, Ortuno-Sahagun D, Camins A, Pallas M. J Pineal Res. 2009 Oct;47(3):228-37.
Cytoplasmic relaxation of active Eph controls ephrin shedding by ADAM10. - Cytoplasmic relaxation of active Eph controls ephrin shedding by ADAM10.
Janes PW, Wimmer-Kleikamp SH, Frangakis AS, Treble K, Griesshaber B, Sabet O, Grabenbauer M, Ting AY, Saftig P, Bastiaens PI, Lackmann M. PLoS Biol. 2009 Oct;7(10):e1000215.