Western blot analysis of lysates from K562, PC-3 cell line and human skeletal muscle tissue lysate(from left to right), using HIST1H2BM Antibody (N-term)(Cat. #AP20679a). AP20679a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
All lanes : Anti-HIST1H2BM Antibody (N-term) at 1:2000 dilution Lane 1: PC-3 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.