Immunohistochemical analysis of paraffin-embedded H. skin section using PITPNA Antibody (C-term)(Cat#AP20431b). AP20431b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: mouse brain lysate Lane 4: MOLT-4 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: rat brain lysate Lane 7: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.