Immunohistochemical analysis of paraffin-embedded H. cerebellum section using GLD2 Antibody (N-term)(Cat#AP5092a). AP5092a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of MCF-7 cells stained with GLD2 Antibody (N-term)(Cat#AP5092a). AP5092a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from mouse brain, mouse cerebellum tissue lysate (from left to right), using GLD2 Antibody (N-term)(Cat. #AP5092a). AP5092a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot analysis of lysates from HepG2 cell line and mouse brain tissue lysate (from left to right), using GLD2 Antibody (N-term)(Cat. #AP5092a). AP5092a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
GLD2 Antibody (N-term) (Cat. #AP5092a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.