Western blot analysis of Panc-1 (lanes 1 and 3) and Jurkat (lanes 2 and 4) cell lysates: 1, 2. Anti-Zinc-Activated Channel (ZAC) (extracellular) antibody (#AG1001), (1:200). 3, 4. Anti-Zinc-Activated Channel (ZAC) (extracellular) antibody, preincubated with the control peptide antigen.
Expression of Zinc-Activated Channel (ZAC) in human (PANC-1) cells Immunocytochemical staining of human intact living epitheioid carcinoma (PANC-1) cells. Extracellular staining (green) of cells with Anti-Zinc-Activated Channel (ZAC) (extracellular) antibody (#AG1001), (1:25) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. DAPI is used as the counterstain (blue).
Indirect flow cytometry analysis of Jurkat living cells: ___ Unstained cells.___ Cells + Anti-Zinc-Activated Channel (ZAC) (extracellular) antibody (#AG1001), (5-10 µg/1x106 cells).