Western blot analysis of rat heart membranes: 1. Anti-Ryanodine Receptor 2 antibody (#AG1394), (1:200). 2. Anti-Ryanodine Receptor 2 antibody, preincubated with the control peptide antigen.
Expression of Ryanodine Receptor 2 in rat cardiac muscle Immunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 antibody (#AG1394), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
Expression of Ryanodine Receptor 2 in mouse cerebellum Immunohistochemical staining of mouse cerebellum frozen sections with Anti-Ryanodine Receptor 2 antibody (#AG1394), (1:100), (green fluorescence). A. The highest expression of Ryanodine Receptor 2 is in the molecular layer (Asterisk) but there is also some expression in the soma of Purkinje cells (arrows). B. In the same section, there is staining for parvalbumin (red), a marker for Purkinje cells. C. Merged image of panels A and B demonstrates that Ryanodine Receptor 2 is localized both in the area surrounding the dendritic tree and in the soma of Purkinje cells. DAPI is used as the counterstain (blue).