All lanes : Anti-ADAM8 antibody [EPR14612] (ab186432) at 1/20000 dilutionLane 1 : THP1 cell lysateLane 2 : KM3 cell lysateLane 3 : Raji cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-ADAM8 antibody [EPR14612] (ab186432) at 1/5000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis on immunoprecipitation pellet from Raji cell lysate, labeling ADAM8 immunoprecipitated using ab186432 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.