All lanes : Anti-ADAMTS15 antibody - Catalytic domain (ab59826) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with SF)Lane 2 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with TPA)Lane 3 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with ILA-beta)Lane 4 : Whole cell lysate prepared from Madin Darby canine kidney (MDCK) cells (treated with TNF-alpha)Lysates/proteins at 30 µl per lane.