All lanes : Anti-ADAMTS4 antibody (ab45038) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with SF) Lane 2 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with TPA) Lane 3 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with IL1-alpha) Lane 4 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with IL1-beta) Lane 5 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with TNA-alpha) Lane 6 : Whole cell lysate prepared from rat osteosarcoma UMR 106 cells (treated with RA)