Anti-Adenosine A1 Receptor antibody [EPR6179]

• Supplier: Abcam
• Catalog: ab124780
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6179
• Applications: WB IP IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues in the extracellular domain of Adenosine A1 Receptor (Human)
• Description: Rabbit Monoclonal
• Gene: ADORA1
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab124780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124780, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

ab124780, at 1/100, staining Adenosine A1 Receptor in formalin fixed paraffin embedded Human breast tissue using immunohistochemistry.

All lanes : Anti-Adenosine A1 Receptor antibody [EPR6179] (ab124780) at 1/1000 dilutionLane 1 : Saos 2 cell lysateLane 2 : SH SY5Y cell lysateLane 3 : Caco 2 cell lysateLane 4 : A549 cell lysateLane 5 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit at 1/2000 dilution

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD