All lanes : Anti-Adenylate Kinase 1 antibody [19G4] (ab117862) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Adenylate Kinase 1 cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-Adenylate Kinase 1 antibody [19G4] (ab117862) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : HT29 cell lysateLane 4 : A549 cell lysateLane 5 : COS7 cell lysateLane 6 : Jurkat cell lysateLane 7 : MDCK cell lysateLane 8 : PC12 cell lysateLane 9 : MCF7 cell lysateLysates/proteins at 35 µg per lane.
HEK293T cells transfected with either RC215130 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab117862, and then analyzed.