Anti-Adiponectin Receptor 1 antibody [EPR6626]

• Supplier: Abcam
• Catalog: ab126611
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6626
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Adiponectin Receptor 1 aa 1-100 (intracellular)
• Description: Rabbit Monoclonal
• Gene: ADIPOR1
• Conjugate: Unconjugated

Product images

Anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µgSecondaryHRP anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human endometrium with purified ab126611 at a dilution of 1/200. The seconday was a HRP polymer for rabbit IgG amd the counter stain used was hematoxylin.

Immunocytochemistry of HeLa cells fixed in 4% PFA with purified ab126611 at a dilution of 1/400. An Alexa Fluor® goat anti-rabbit antibody was used as the secondary and the sample was counter stained wtih DAPI.

All lanes : Anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) at 1/1000 dilution (Purified)Lane 1 : Mouse heartLane 2 : Rat heartLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Overlay histogram showing HUVEC cells stained with purified ab126611 (pink line) at a dilution of 1/300. The cells were fixed with 2% PFA). The secondary antibody used was FITC goat anti-rabbit IgG and rabbit monoclonal IgG was used as the isotype control.

Immunohistochemical staining of Adiponectin Receptor 1 in formalin-fixed, paraffin-embedded human kidney tissue by unpurified ab126611, at 1/50 dilution.

All lanes : Anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) at 1/1000 dilution (Unpurified)Lane 1 : Human muscle lysateLane 2 : Human heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-rabbit HRP at 1/2000 dilution

Overlay histogram showing HepG2 cells stained with unpurified ab126611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126611, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Equilibrium disassociation constant (KD) with unpurified ab126611.Learn more about KD Click here to learn more about KD

Product References

Effects of bariatric surgery on human small artery function: evidence for - Effects of bariatric surgery on human small artery function: evidence for

Aghamohammadzadeh R, Greenstein AS, Yadav R, Jeziorska M, Hama S, Soltani F, Pemberton PW, Ammori B, Malik RA, Soran H, Heagerty AM. J Am Coll Cardiol. 2013 Jul 9;62(2):128-35.