All lanes : Anti-Advillin antibody (ab72210) at 1 µg/mlLane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Skin (Mouse) Whole Cell Lysate - normal tissue (ab4025)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab72210 staining Advillin in murine sciatic nerve tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in formaldehyde, blocked with 3% BSA for 45 minutes and then incubated with ab72210 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.See Abreview