Anti-AFG3L2 antibody

• Supplier: Abcam
• Catalog: ab68023
• Prices: $370.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Human
• Antigen: Full length protein (Human)
• Description: Mouse Polyclonal
• Gene: AFG3L2
• Conjugate: Unconjugated

Product images

All lanes : Anti-AFG3L2 antibody (ab68023) at 1/500 dilutionLane 1 : AFG3L2 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilutiondeveloped using the ECL technique

ICC/IF image of ab68023 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68023, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity - Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity

MacVicar TD, Lane JD. J Cell Sci. 2014 May 15;127(Pt 10):2313-25.

Activation of mitochondrial protease OMA1 by Bax and Bak promotes cytochrome c - Activation of mitochondrial protease OMA1 by Bax and Bak promotes cytochrome c

Jiang X, Jiang H, Shen Z, Wang X. Proc Natl Acad Sci U S A. 2014 Oct 14;111(41):14782-7. doi: